Journal: bioRxiv
Article Title: Lack of Oncomodulin Increases ATP-Dependent Calcium Signaling and Susceptibility to Noise in Adult Mice
doi: 10.1101/2024.06.10.598303
Figure Lengend Snippet: (A) qRT-PCR results of P2RX2 relative expression, cochleae are taken from 3 - 4 wks Ocm +/+ and Ocm -/- mice with or without noise exposure. Total mRNA was extracted immediately after dissection. n ≥ 3 for each genotype under each experimental condition. All values were normalized to pre-noise Ocm +/+ . mean ± SD, p < 0.05, two-way ANOVA (B) P2X2 immunofluorescence shows the localization of P2X2 (white) in the organ of Corti. The mid-modiolar sections of cochleae were harvested from pre-noise Ocm +/+ and Ocm -/- mice at 3 - 4 wks of age. The red dashed box represents the OHC region, the red arrows point to the top of the OHC, and the yellow arrows show adjacent Deiters’ cells. P2X2 receptor labeling was in the cell body of supporting cells and the straight phalangeal processes (yellow arrows). TM: tectorial membrane. (C) Maximum intensity projections of P2X2 immunolabeling on three rows of OHCs harvested from pre- and post-noise Ocm +/+ and Ocm -/- mice. P2X2 (white), GCaMP6s (green), and DAPI (blue) are shown. Red arrows point to the OHC stereocilia. (D, E) Representative western blot for P2X2 protein expression levels detected in single cochlea derived from pre- or post-noise Ocm +/+ and Ocm -/- mice at 3 - 4 wks. n = 3 for each genotype under each experiment condition, β-tubulin (loading control) was used for normalization, all values were then normalized to pre-noise Ocm +/+ . p < 0.05 two-way ANOVA , ns: not significant, * : p < 0.05; ** : p < 0.01, Bonferroni post-test.
Article Snippet: Samples were stained with antibodies to P2X2 (Alomone Labs, Israel, APR-003, 1:400).
Techniques: Quantitative RT-PCR, Expressing, Dissection, Immunofluorescence, Labeling, Membrane, Immunolabeling, Western Blot, Derivative Assay, Control
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